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Erro
  • JFTP::login: Unable to login
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  • JFTP::delete: Bad response
  • JFTP::delete: Bad response
  • JFTP::delete: Bad response
  • JFTP::delete: Bad response
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Stress Biótico: resposta radicular à infecção por Phytophthora cinnamomi

 

Resumo

In Phytophthora, infection generally starts when motile zoospores released from sporangia reach a leaf or root surface, encyst, and germinate. Adhesion of cysts to plant surfaces occurs rapidly following zoospore encystment. The cysts germinate, and the germ tubes swell to form appressorium-like structures that facilitate adhesion and penetration of plant surfaces. In root-infecting species, penetration can occur in between cells without the aid of an appressorium. Penetration and colonization of host tissue involves the secretion of a range of degradative enzymes that break down physical barriers to infection. Suppression of host defences can occur through the production of inhibitory proteins that target host enzymes. P. cinnamomi can form chlamydospores, resistance structures that allow its survival in soil or decaying plant material, in a dormancy state. When conditions favouring growth prevail, the pathogen enters the asexual sporulation cycle, generating motile zoospores or produces hyphae that infect directly host roots.

RNA will be extracted from roots of two different types of cork oak plants: 1) three years old plants and 2) recently germinated from seeds. Roots from recently germinated acorns will be artificially infected with zoospores and three years-old plant roots will be infected with chlamydospores, in order to reproduce natural infections processes. Non-infected roots of each type will be used as control. Due to limitations at this stage of the funding programme, ESTs generated in infection conditions will be pooled for each root type in order to have all mRNA differentially expressed at different time points post challenge represented (8, 20 and 36 h for roots germinated from acorns, and 3 months for 3 years old plants). RNA from each type root will be composed of a pool from several specimens. This pool will consist of individuals from populations of different geographic regions of Portugal and Spain (Algarve, Alto-Alentejo, Trás-os-Montes, Andalucia) in order to take into account genetic diversity among and within populations and generate a more representative cDNA library of the species, increasing the probabilities of finding different isoforms of the same gene.

 

 

Grupos Envolvidos:

Laboratório de Biotecnologia Molecular e Fitopatologia

Plant and animal genomic group

 

Membros:

Alfredo Cravador
Ana Cristina Coelho
Marília Horta

 

Publicações Relacionadas:

Involvement of the β-cinnamomin elicitin in infection and colonisation of cork oak roots by Phytophthora cinnamomi. M. Horta, P. Caetano, C. Medeira, I. Maia and A. Cravador, European Journal of Plant Pathology 127 (3) Page 427-436 (2010). DOI: 10.1007/s10658-010-9609-x.


Maia, I., Medeira, C., Candeias, I.; Moreira, A., Melo, E. and Cravador, A. Exposition of cork oak roots to cryptogein reduced the infection by Phytophthora cinnamomi. Microscopy and Microanalysis 15 (supp 3), 27-28, 2009.


M. Horta; N. Sousa; A. C. Coelho; D. Neves and A. Cravador. In vitro and in vivo quantification of elicitin expression in Phytophthora cinnamomi, Physiological and Molecular Plant Pathology, 73, 48–57 (2008).


Maia, I.; Medeira, C.; Melo, E.; Cravador, A. Quercus suber infected by Phytophthora cinnamomi. Effects at cellular level of cinnamomin on roots, stem and leaves. Microscopy and Microanalysis, 14 (supp 3), 146-147 (2008)

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Coelho, A. C., Horta, M., Neves, D. and Cravador, A. Involvement of a cinnamyl alcohol dehydrogenase of Quercus suber in the defence response to infection by Phytophthora cinnamomi. Physiological and Molecular Plant Pathology, 69 62–72 (2006).


Coelho, A.C.; Lima, M.B.; Neves, D. and Cravador, A. Genetic diversity of two evergreen oaks (Quercus suber L. and Q (ilex) rotundifolia Lam.) in Portugal using AFLP markers. Silvae Genetica 55(3), 105-118 (2006).


J. Duclos, A. Fauconnier, A.C. Coelho, A. Bollen, A. Cravador and E. Godfroid. Identification of an elicitin gene cluster in Phytophthora cinnamomi; DNA Sequence 67, 231-237 (1998).


C. Coelho, A. Cravador, A. Bollen, J.F.P. Ferraz, A.C. Moreira, A. Fauconnier and E.Godfroid. Highly specific and sensitive non-radioactive molecular identification of Phytophthora cinnamomi; Mycological Research, 101 (12): 1499-1507 (1997).

 

Instituições Envolvidas:

UAlg

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